Anti-iNOS/NOS II; NT

Code: ABN26 D2-231

Analysis Note

ControlIFNgamma/LPS untreated and treated RAW264.7 cell lysates

Application

Immunohistochemistry Analysis: 1:50-200 dilution from a...


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Analysis Note

ControlIFNgamma/LPS untreated and treated RAW264.7 cell lysates

Application

Immunohistochemistry Analysis: 1:50-200 dilution from a representative lot detected iNOS/NOS II in malignant human lung tissues.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated iNOS/NOSII from IFNgamma/LPS treated RAW264.7 cell lysate.

Research CategoryNeuroscience

Detect iNOS/NOS II using this Anti-iNOS/NOS II Antibody, NT validated for use in WB, IP, IH(P).

Research Sub CategoryOxidative Stress

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Immunogen

Epitope: N-terminus

GST-tagged recombinant protein corresponding to the N-terminus of mouse iNOS/NOS II.

Linkage

Replaces: 06-573

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in IFNgamma/LPS untreated and treated RAW264.7 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected iNOS/NOS II on 10 µg of IFNgamma/LPS untreated and treated RAW264.7 cell lysates.

Specificity

This antibody recognizes iNOS/NOS II at the N-terminus.

Other homologies: Rat (85% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~125 kDa observed

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationmouse ... Nos2(18126)
NCBI accession no.NP_035057
packagingantibody small pack of 25 µg
purified byaffinity chromatography
Quality Level100
shipped inambient
species reactivitymouse, human
storage temp.2-8°C
technique(s)western blot: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable
UniProt accession no.P29477
This product has met the following criteria to qualify for the following awards:



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